Euglena Medium

Euglena Medium Suitable for most axenic cultures of Euglena and Chlorogonium. Directions For 1 L Total To approximately 950 mL of dH2O, add each of the components in the order specified while stirring continuously. Bring the total volume to 1 L with dH2O. For 1.5% agar medium add 15 g of agar into the flask; do not mix. Cover and autoclave medium. Store at refrigerator temperature. # Component Amount Stock Solution Concentration Final Concentration 1 Sodium acetate(Fisher BP 333) 1 g/L 12.2 mM 2 Beef extract(Sigma B-4888) 1 g/L 3 Tryptone(Sigma T 9410)) 2 g/L 4 Yeast extract(Bacto, Difco) 2 g/L 5 CaCl2•2H2O(Sigma C-3881) 0.01 g/L 0.07 mM