LDM Medium

LDM Medium Modification of Lewin's marine diatom medium. Directions For 1 L Total To approximately 950 mL of seawater (30 ppt), add each of the components in the order specified (except vitamins) while stirring continuously. Bring total volume to 1 L with non-pasteurized seawater. For 1.5% (w/v) agar medium, add 15 g of agar into the flask; do not mix. Cover and autoclave medium. When cooled add sterile vitamins. For agar medium add vitamins, mix, and dispense before agar solidifies. Store at refrigerator temperature. # Component Amount Stock Solution Concentration Final Concentration 1 Seawater (30 ppt) 950 mL 2 NaNO3(Fisher BP360-500) 1 mL/L 10 g/400mL dH2O 0.29 mM 3 CaCl2•2H2O(Sigma C-3881) 1 mL/L 1 g/400mL dH2O 0.017 mM 4 MgSO4•7H2O(Sigma 230391) 1 mL/L 3 g/400mL dH2O 0.03 mM 5 K2HPO4(Sigma P 3786) 1 mL/L 3 g/400mL dH2O 0.043 mM 6 KH2PO4(Sigma P 0662) 1 mL/L 7 g/400mL dH2O 0.129 mM 7 NaCl(Fisher S271-500) 1 mL/L 1 g/400mL dH2O 0.043 mM 8 P-IV Metal So