Modified 2X CHEV + Soil Medium

Modified 2X CHEV + Soil Medium The medium is prepared on a tube by tube basis, using 15 mL glass tubes, stoppered with rubber-lined screw caps. Directions Add the components in the following order. Once soil and liquid are mixed, the media must be processed (pasteurized or autoclaved) within 6 hrs. For routine maintenance and transfer, autoclaved media for 15 min, since transfer material typically contains organics and/or bacteria needed for algal growth. In pasteurization, tubes are brought to just over 98 °C, and held at that temperature for 2 hrs. They are then allowed to cool to room temperature for 20-24 hrs. This process is repeated for three successive days. It is imperative that media (pasteurized or autoclaved) be allowed to gas-equilibrate for 2 days prior to use. # Component Amount Stock Solution Concentration Final Concentration 1 CR1 Soil 1 cc 2 MgCO3 (MCIB CB486) 0.5 mg/12 mL 0.5 mM 3 dH2O 10 mL 4 F/2 Medium 2 mL