Modified Artificial Seawater Medium

Modified Artificial Seawater Medium Modification of artificial seawater medium for B 2795 Picochlorum oklahomensis. Directions For 1 L Total To approximately 850 mL of dH2O, add each of the components in the order specified (except vitamins) while stirring continuously. Adjust the pH to 8.0. Bring the total volume to 1 L with dH2O. For 1.5% agar medium add 15 g of agar into the flask; do not mix. Cover and autoclave medium. When cooled add vitamins. For agar medium add vitamins, mix, and dispense before agar solidifies. Store at refrigerator temperature. # Component Amount Stock Solution Concentration Final Concentration 1 NaCl(Fisher S271-500) 30 g/L 0.51 M 2 MgSO4•7H2O(Sigma 230391) 10 mL/L 24.4 g/100 mL dH20 9.9 mM 3 KCl(Fisher P 217) 10 mL/L 6 g/100 mL dH20 8 mM 4 NaNO3(Fisher BP360-500) 10 mL/L 10 g/100 mL dH20 11.8 mM 5 CaCl2•2H2O(Sigma C-3881) 10 mL/L 3 g/100 mL dH20 2.04 mM 6 KH2PO4(Sigma P 0662) 10 mL/L 0.5 g/100 mL dH20 0.37 mM 7 Tricine(Sigma T-5816-256) 10 mL/L 10 g/100 mL dH20 5.58 mM 8 NH4Cl(Fisher A 649-500) 1 mL/L 2.7 g/100 mL dH20 0.5 mM 9 P-IV Metal Solution 1 mL/L 10 Soilwater: GR+ Medium 30 mL/L 11 Vitamin B12 1 mL/L 12 Biotin Vitamin Solution 1 mL/L 13 Thiamine Vitamin Solution 1 mL/L