Modified Desmidiacean Medium

Modified Desmidiacean Medium The final medium is prepared on a tube by tube basis, using 15 mL glass tubes, stoppered with rubber-lined screw caps. Directions For 1 L Total To approximately 900 mL of dH2O add the first 7 components in the order specified while stirring continuously. Bring total volume to 1 L with dH2O. Cover and autoclave medium. Allow to cool. Add 1 sterile barley grain per test tube then store at refrigerator temperature. # Component Amount Stock Solution Concentration Final Concentration 1 KNO3(Baker 3190) 10 mL/L 1 g/100 mL 0.9 mM 2 (NH4)2HPO4(Fisher A686) 5 mL/L 0.2 g/100 mL 0.075 mM 3 MgSO4•7H2O(Sigma 230391) 10 mL/L 0.1 g/100 mL 3.3 µM 4 CaSO4•2H2Osaturated solution (Mallinckroft 4300) 10 mL/L 5 Soilwater: GR+ Medium 20 mL/L 6 Soilwater: Peat Medium 10 mL/L 7 MWC Metal Solution 5 mL/L 8 Barley grains autoclaved 1 grain/10 mL