1/4 Soil+Seawater Medium

1/4 Soil+Seawater Medium For some marine and brackish-water algae requiring salinities at 7.5 ppt, glass-distilled water and pasteurized, filtered seawater are combined prior to steaming, such that 1/4 Soil+Seawater contains seawater combined with distilled water 1:3. Directions For 200 mL Total Combine all components. Cover the medium container and steam for 2 consecutive days, 3 hours on each day. Pasteurization is a gradual rising of temperature to approximately 95 °C in 15 minutes. Then increased just over 98 °C for the 3 hour duration. Cooling occurs gradually at room temperature. Refrigerate 24 hours or more and bring to room temperature before using. # Component Amount Stock Solution Concentration Final Concentration 1 Green House Soil 1 tsp 2 CaCO3 (optional) (Fisher C 64) 1 mg 0.05 mM 3 Pasteurized Seawater 50 mL 4 dH2O 150 mL