Snow Algae Medium

Snow Algae Medium Suitable for snow algae collection. Directions For 1 L Total To approximately 500 mL of dH2O, add each of the components in the order specified while stirring continuously. Bring the total volume to 1 L with dH2O, pH is 5.1-5.3. For 1.5% agar medium add 15 g of agar into the flask; do not mix. Cover and autoclave medium. Store at refrigerator temperature. # Component Amount Stock Solution Concentration Final Concentration 1 CaCl2•2H2O(Sigma C-3881) 1 mL/L 0.5 g/100 mL dH2O 0.03 mM 2 NaNO3(Fisher BP360-500) 1 mL/L 2.5 g/100 mL dH2O 0.29 mM 3 NH4Cl(Fisher A 649-500) 1 mL/L 0.5 g/100 mL dH2O 0.093 mM 4 CaSO4•2H2O(Mallinckroft 4300) 1 mL/L 0.4 g/100 mL dH2O 0.023 mM 5 MgSO4•7H2O(Sigma 230391) 1 mL/L 0.5 g/100 mL dH2O 0.02 mM 6 Na2SiO3•9H2O(Sigma 307815) 1 mL/L 0.2 g/100 mL dH2O 0.007 mM 7 FE EDTA 250 mL/L 8 Minor Nutrients 10 mL/L 9 K2HPO4(Sigma P 3786) 2 mL 3.48 g/100 mL dH2O 0.4 mM 10 KH2PO4(Sigma P 0662) 98 mL 2.72 g/100 mL dH2O 0.02 M