Soil Extract + Sodium Metasilicate Medium

Soil Extract + Sodium Metasilicate Medium Modification of Soil Extract Medium for diatoms. Directions For 1 L Total Prepare 1 L of Bristol Medium and thoroughly mix. Discard 40 mL of the Bristol Medium. Add 40 mL of previously prepared GR+ Medium [Note: The GR+ should be filtered to remove soil particles]. For 1.5% agar medium add 15 g of agar into the flask; do not mix. Cover and autoclave medium. When cooled, add filter sterilized sodium metasilicate. For agar medium, add sodium metasilicate, mix, and dispense before agar solidifies. Store at refrigerator temperature. # Component Amount Stock Solution Concentration Final Concentration 1 Bristol Medium 960 mL 2 Soilwater: GR+ Medium 40 mL of supernatant 3 Sodium Metasilicate 1 mL 200 mM 200 µM