Soilwater: GR+ Medium

Soilwater: GR+ Medium The basic garden-type soilwater; includes a pinch of CaCO3, which is added to the soil and water prior to steaming; suitable for most phototrophic freshwater algae. Optional ingredients: add Vitamin B12 to cultures of Volvox; a pinch of NH4MgPO4•6H2O added to soilwater cultures of Botryococcus, Synechococcus and some Euglenoids enhances growth and to LB 826 Gonium pectorale increases the numbers of 16-celled colonies formed. Directions For 200 mL Total Combine components. Cover the medium container and steam for 2 consecutive days, 3 hours on each day. Pasteurization is a gradual rising of temperature to approximately 95 °C in 15 minutes. Then increased just over 98 °C for the 3 hour duration. Cooling occurs gradually at room temperature. Refrigerate 24 hours or more and bring to room temperature before use. # Component Amount Stock Solution Concentration Final Concentration 1 Green House Soil 1 tsp 2 CaCO3(optional)(Fisher C 64) 1 mg 0.05 mM 3 dH2