5-alpha Chemically Competent E. coli Cells for Cloning and Subcloning.

AB 5-alpha chemically competent E.coli cells are comparable to the DH5α™ strain of E. coli. AB 5-alpha cells carry recA1 and endA1 mutations that minimize recombination and improve plasmid stability and quality. They also contain a lacZ marker that provides α-complementation of the β-galactosidase gene for blue/white color screening and are ideal for cloning, sub-cloning, library construction, and plasmid isolation. The transformation efficiency is  ≥ 1x108 cfu/μg pUC19 plasmid DNA. AB 5-alpha chemically competent cells are supplied as a pack of 10 convenient 100 μl/tube aliquots. AB 5-alpha electrocompetent cells are also available.  Catalog # AB5-201 E. coli genotype: F- endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+) deoR supE44 phoA Δ(lacZYA-argF)U169 Φ80lacZΔM15 λ– thi-1   Features & Benefits Comparable to DH5α™ Efficient transformation of cloned DNA into bacterial cells Blue/white color selection Economical price Storage:  at -80°C. Thaw on ice only before use. Do not re-freeze.