Lambda Protein Phosphatase for Dephosphorylation of Proteins

Lambda Protein Phosphatase (Lambda PP) is a Mn2+-dependent protein phosphatase with activity towards phosphorylated serine, threonine and tyrosine residues (1, 2). It is the 221 amino-acid product of the ORF221 open reading frame on bacteriophage lambda (1). The enzyme is inhibited by vanadate ions and can be heat inactivated at 65° C for 1 hour in the presence of 50 mM Na2EDTA (3). Amid Biosciences recombinant full-length Lambda Protein Phosphatase with a C-terminal His-tag expressed in E. coli and purified to >90% homogeneity (SDS-PAGE) with no detectable protease, DNase or RNase activity. Molecular Weight: 25 kDa Specific activity: ~600,000 units/mg Unit Definition: One unit will hydrolyze 1 nmol of p-nitrophenyl phosphate (pNPP) in 1 minute at 30° C and pH 7.5 in a total reaction volume of 50 μl. Catalog# LPP-301-20, LPP-301-100 This product is intended for laboratory research use only. Recommended Usage Lambda Phosphatase can be used to release phosphate groups from phosphoryla