Rhamnose-Inducible Expression Vector - pTrham

Amid Biosciences’ pTrham vector produces the highest yields of recombinant proteins in comparison to other rhamnose expression vectors available on the market. To ensure highly efficient transcription and translation of the cloned gene, L-rhamnose-inducible  rhaBAD promoter of Escherichia coli is complemented with the rrnG anti-termination region, the bacteriophage T7 gene 10 translation enhancer and ribosome binding site.  This plasmid-based expression system with optimized transcription and translation regulatory elements is capable of efficient protein production upon induction with L-rhamnose but exhibits very low basal levels of expression in the absence of inducer. pTrham expression is tightly regulated at the individual cell level and promotes the high-level production of heterologous proteins in a correct and bioactive conformation. As a result, pTrham vector is capable of expressing a wide variety of recombinant proteins, including the stable cloning and expression of toxic pr