Taq DNA Polymerase
Taq DNA Polymerase is a thermostable DNA polymerase for everyday routine PCR. Taq DNA Polymerase possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The enzyme lacks 3´ → 5´ exonuclease (proofreading) activity. Taq DNA polymerase’s terminal transferase activity of adding a single nucleotide (adenosine) at 3' end of the extension product allows TA cloning of PCR products. Products up to 5 kb can be amplified from templates including genomic and plasmid DNA, bacterial colonies, and cDNA products. It is supplied with 10X Taq Reaction Buffer. Features and benefits Isolated from a recombinant source Non-proofreading DNA Polymerase Tolerates a wide range of templates Suitable for primer extension and TA cloning Robust and reliable performance at low price Catalog # PC-TDP-601 10 X Reaction Buffer (with Mg2+):100 mM Tris HCl (pH 8.3 at 25°C), 500 mM KCl, 15 mM MgCl2,Concentration:Taq Polymerase is supplied as 5 units/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA