Biotinylated Human TIGIT Protein, Active

Product Overview Description Recombinant Biotinylated Human TIGIT Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met22-Pro141. Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC Accession Q495A1 Target Symbol TIGIT Synonyms TIGIT; VSIG9; VSTM3; WUCAM Species Human Expression System HEK293 Tag C-His-Avi Expression Range Met22-Pro141 Mol. Weight The protein has a predicted MW of 16.1 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result. Form Lyophilized Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Endotoxin Less than 1EU per ug by the LAL method. Activity Immobilized Biotinylated Human TIGIT, His-Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD155, hFc Tag with the EC50 of 11.7ng/ml determined by