E. coli "6AL" for proteins with disulfides

Product Description Roke’s 6AL E. coli strain is a proprietary bacterial strain that has been engineered to express high titers of nanobodies while also providing cell autolysis and nucleotide autohydrolysis capabilities to facilitate routine downstream workflows. Our strain leverages two-stage protein expression where cells grow until phosphate in the media becomes depleted. Once low phosphate conditions are reached, expression of the protein of interest is driven by the activation of low phosphate inducible promoters. Upon entry into stationary phase, targeted depletion of TrxB changes the redox cytoplasmic potential facilitating the formation of disulfide bonds. In addition to the protein of interest, two chromosomally integrated accessory proteins are co-expressed: a lysozyme expressed in the cytosol (λR gene from bacteriophage lambda) and a nuclease expressed in the periplasm (nucA gene from Serratia marcescens). After cell harvesting, addition of a low amount of detergent (0.1% v