Pfu Fusion DNA polymerase

Description This high fidelity DNA Polymerase is a thermostable enzyme engineered from Pyrococcus furiosus, by fusing it with the small, non‑specific DNA‑binding protein Sso7d from Sulfolobus solfataricus. This fusion significantly enhances the enzyme’s processivity and performance while preserving its 5′→3′ polymerase activity and intrinsic 3′→5′ proofreading exonuclease activity. As a result, Pfu‑sso7d generates highly accurate PCR products with substantially reduced error rate (>30x less errors than Taq DNA polymerase), making it an ideal choice for cloning, site‑directed mutagenesis, and other applications that demand low-error DNA amplification, even from complex or GC‑rich templates. This product includes all reagents needed to perform a PCR reaction with the exception of dNTPs, primers and DNA template. It includes: Component Composition Pfu-sso7d DNA Polymerase 1 units/ul 5x Pfu polymerase buffer 50 mM Tris-HCl, pH 8.5, 250 mM KCl, 7.5 mM MgCl2, 50 mM (NH4)2SO4, 0.