Aequorin Expressed in Mitochondria Cells Human Umbilical Vein Endothelial Cells (HUVECs-AEQ-Mito)

Aequorin Expressed in Mitochondria Cells Human Umbilical Vein Endothelial Cells (HUVECs-AEQ-Mito) Catalogue Number: UBP01AEQ Product Format: Frozen Vial or T-25 flask Cell Number: > 1x10^6 cells/vial Description:HUVECs are isolated from the normal human umbilical vein and HUVECs-AEQ-Mito cells are specifically selected from HUVECs infected with lentiviruses expressing Chimiral GFP-Aequorin in the mitochondria, using puromycin. The cells are shipped in frozen vials (provided at passage 2). Universal Endothelial Growth Medium, containing 10% serum and growth supplements, is recommended for cell culture. These cells have a minimum average population doubling level of > 10 when cultured following the detailed protocol described below. Characterization of the Cells:1. Cytoplasmic VWF/Factor VIII >95% positive by immunofluorescence2. Cytoplasmic uptake of DiI-Ac-LDL >95% positive by immunofluorescence3. Cytoplasmic PECAM1 >95% positive by immunofluorescence4. HUVECs are negati