Human Brain Microvascular Endothelial Cells Expressing GFP-VE-CAD
Human Brain Microvascular Endothelial Cells Expressing GFP-VE-CAD - Catalogue Number: UBP02VE-CAD-GFP- Product Format: Frozen Vial- Cell Number: Each vial contains over 5x10[5] cells Let's delve into the details! These exceptional HBMVEC-GFP-VE-CAD cells were isolated from the normal human cerebral cortex and carefully selected by puromycin from HBMVECs after being infected with special lentiviruses that express GFP-VE-CAD. The cells, provided at passage 2, are shipped in frozen vials and boast a remarkable minimum population doubling level of >15 when following the provided detailed protocol. Characterization of the Cells:1. Over 95% positive for cytoplasmic VWF/Factor VIII by immunofluorescence2. Over 95% positive for cytoplasmic uptake of Di-I-Ac-LDL by immunofluorescence3. Over 95% positive for cytoplasmic PECAM1 by immunofluorescence4. HBMVECs are negative for HIV-1, HBV, HCV, and mycoplasma For optimal cell culture, we recommend using our Universal Endothelial Growth Medium co