2X Erdschreiber's Medium
$55.00
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2X Erdschreiber's Medium Erdschreiber's Medium made with Supplemented Seawater instead of Pasteurized Seawater. Directions For 3 L Total To 3 L of pasteurized supplemented seawater (60 ppt) aseptically add each of the sterile components in the order specified. Vigorously swirl the contents of the flask to mix thoroughly. Store at refrigerator temperature. # Component Amount Stock Solution Concentration Final Concentration 1 Supplemented Seawater 3 L 2 P-IV Metal Solution 36 mL/3 L 3 NaNO3(Fisher BP360-500) 10 mL/3 L 0.7 M 2.3 mM 4 Na2HPO4•7H2O(Sigma S-9390) 10 mL/3 L 0.02 M 0.067 mM 5 Soilwater: GR+ Medium 150 mL/3 L 6 Vitamin B12 3 mL/3 L
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