Hieff™ Taq DNA Polymerase _ 10101ES

Hieff™ Taq DNA polymerase is a thermostable recombinant wild type DNA polymerase expressed by Thermus aquaticus with a molecular weight of 94 KDa. It has 5'→3' polymerase activity and 5'→3' exonuclease activity but without 3'→5' exonuclease activity. The amplified product has 3'- dA and can be directly used for TA cloning. Features 5'→3' DNA polymerase activity 5'→3' exonuclease activity Hieff™ Taq DNA polymerase is an ideal tool for standard PCR with templates of 5 kb or less Applications Genotyping, colony PCR and other conventional PCR Specifications Polymerase Taq DNA polymerase Molecular Weight 94 KDa Unit Definition Using the activated DNA of salmon sperm as template/primer, the activity is defined as one active unit (U) when 10 nmol of total nucleotide was ingested as acid insoluble substance at 74℃ for 30 min. Components Components No. Name 10101ES80 (1, 000 U) 10101ES92 (10, 000 U) 10101-A 10×Taq Buffer (Mg2+ Free) 4×1 mL 4×10 mL 10101-B 25 mM