Recombinant Deoxyribonuclease I (DNase I, RNase-free) (2 U/μL) -10325ES
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Recombinant Deoxyribonuclease I (DNase I, RNase-free) (2 U/μL) -10325ES

$55.00
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DNase I is an endonuclease that can digest single- or double-stranded DNA. It can hydrolyze phosphodiester bonds to produce mono- and oligodeoxynucleotides containing a 5'-phosphate group and a 3'-OH group.The optimal working pH range of DNase I is 7-8. The activity of DNase I depends on Ca2+ and can be activated by divalent metal ions such as Co2+, Mn2+, Zn2+, etc. In the presence of Mg2+, DNase I can randomly cleave any site of double-stranded DNA; while in the presence of Mn2+, DNase I can cleave DNA double-stranded at the same site, forming blunt ends or sticky ends with 1-2 nucleotides protruding. It can be used for the processing of various RNA samples. Features Recombinant source. RNase-free. High enzymatic cleavage efficiency. More suitable for applications sensitive to RNase. Applications Removal of gDNA before RNA extraction or reverse transcription. Removal of template DNA in in vitro transcription. rRNA removal in RNA library construction and sequencing. Nick translatio

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