Biotinylated Human IL-25/IL-17E Protein, His-Avi Tag

Specifications Synonyms IL17E;IL-25; interleukin-25; Interleukin-17E Source Biotinylated Human IL-25/IL-17E Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Tyr33-Gly177.[Accession | Q9H293-1] Molecular Weight The protein has a predicted MW of 19.24 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC Activity ELISA Data: Immobilized Human IL-17RB, His Tag at 5μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human IL-25, His Tag with the EC50 of 58.5ng/ml determined by ELISA. Formulation Supplied as 0.22 μm filtered solution in PBS (pH 7.4).   Storage The product should be stored at -85~-65℃ for 1 year from date of receipt. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Note