Biotinylated Human FGFR3 alpha (IIIb) Protein, His-Avi Tag

Specifications Synonyms CEK; CD333;ACH; EC 2.7.10 Source Biotinylated Human FGFR3 (IIIb) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu23-Gly377.[Accession | P22607-2] Molecular Weight The protein has a predicted MW of 41.4 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Anti-FGFR3 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FGFR3 alpha (IIIc) , His Tag with the EC50 of 35.9ng/ml determined by ELISA. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve t