Biotinylated Human FGFR2 beta (IIIb) Protein, His-Avi Tag

Specifications Synonyms KGFR;FGFR-2;Fibroblast growth factor receptor 2;Keratinocyte growth factor receptor Source Biotinylated Human FGFR2 beta (IIIb) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Arg152-Glu378.[Accession | P21802-3] Molecular Weight The protein has a predicted MW of 28.3 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Anti-FGFR2 IIIb Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FGFR2 beta (IIIb) , His Tag with the EC50 of 13.1ng/ml determined by ELISA. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting