Biotinylated Human FGFR3 beta (IIIc) Protein, His-Avi Tag

Specifications Source Recombinant Biotinylated Human FGFR3 beta (IIIc) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Asp127-Gly377.[uniprot  | P22607] Molecular Weight The protein has a predicted MW of 30.1 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by Tris-Bis PAGE and HPLC Activity ELISA Data: Immobilized Anti-FGFR3 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FGFR3 beta (IIIc) , His Tag with the EC50 of 37ng/ml determined by ELISA. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein