Biotinylated Human Fc gamma RIIB/CD32b Protein, His-Avi Tag

Specifications Source Recombinant Biotinylated Human Fc gamma RIIB/CD32b Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ala46-Pro217. [Uniprot | P31994-1] Molecular Weight The protein has a predicted MW of 22.5 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on Tris-Bis PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by Tris-Bis PAGE and HPLC Activity SPR Data: Biotinylated Human Fc gamma RIIB, His-Avi Tag captured on CM5 Chip via Anti-his antibody can bind Rituximab with an affinity constant of 5.87 μM as determined in SPR assay. SPR Data: Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIB, His-Avi Tag with an affinity constant of 3.97 μM as determined in SPR assay  Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. R