Biotinylated Human M-CSF/CSF-1 Protein, His-Avi Tag

Specifications Source Biotinylated Human M-CSF/CSF-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu33-Asn190.[Accession | P09603-1] Molecular Weight The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 27-37 kDa based on Tris-Bis PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by Tris-Bis PAGE and HPLC Activity ELISA Data: Immobilized Human M-CSF R, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human M-CSF, His Tag with the EC50 of 19.4ng/ml determined by ELISA.  Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.   Stor