Biotinylated Human DLL4 Protein, His-Avi Tag _ 93875ES

Specification Synonyms notch ligand DLL4; MGC126344;DLL4; Delta-like 4 Source Biotinylated Human DLL4 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ser27-Pro524.[Accession | Q9NR61] Molecular Weight The protein has a predicted MW of 55.7 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Anti-DLL4 Antibody, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human DLL4, His Tag with the EC50 of 0.21μg/ml determined by ELISA. Formulation Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve th