Biotinylated Human DLL3 Protein (Primary Amine Labeling), His Tag

Specifications Synonyms Pudgy; DLL3;Delta3; SCDO1delta3 Source Biotinylated Human DLL3 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the N-Terminus. It contains Ala27-Arg490.[Accession | Q9NYJ7-1] Molecular Weight The protein has a predicted MW of 50.4 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Anti-DLL3 Antibody, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human DLL3, His Tag with the EC50 of 18.7ng/ml determined by ELISA. Formulation Lyophilized from 0.22μm filtered solution in PBS, 200mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended.