Biotinylated Human IL-6R alpha/CD126 Protein, His-Avi Tag

Specifications Source Biotinylated Human IL-6 R alpha/CD126 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Leu20-Pro365.[Accession | P08887-1] Molecular Weight The protein has a predicted MW of 41.4 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by Tris-Bis PAGE and HPLC Activity ELISA Data:Immobilized Human IL-6, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-6 R alpha, His Tag with the EC50 of 1.04μg/ml determined by ELISA.   Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.