Biotinylated Human NKp30/NCR3/CD337 Protein, His-Avi Tag

Specifications Synonyms NCR3; LY117;CD337; 1C7 Source Biotinylated Human NKp30/NCR3/CD337 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Leu19-Thr138.[Accession | AAH52582] Molecular Weight The protein has a predicted MW of 20 kDa. Due to glycosylation, the protein migrates to 26-45 kDa based on SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Biotinylated Human NKp30, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-NKp30 Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA . Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommen