Recombinant Human Insulin Protein

Description Recombinant Human Insulin Protein, produced in E. coli, is a two chain, non-glycosylated polypeptide hormone containing 51 amino acids and has a molecular mass of 5807 Dalton. Insulin is purified by proprietary chromatographic techniques. Summary Insulin is a peptide hormone produced by beta cells of the pancreatic islets encoded in humans by the INS gene. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver (UniProtKb_P01308). Recombinant Human Insulin is a commonly used media supplement in mammalian cell culture. CAS Registry No. 11061-68-0 Purity Purity greater than 98.0% as determined by RP-HPLC analysis. Biological Activity The biological activity was determined to be 28 units/mg. Formulation Sterile-filtered white lyophilized (freeze-dried) powder. The protein was lyophilized from a concentrated (1 mg/ml) so