1,25-Dihydroxyvitamin D3 ELISA Kit

1,25-Dihydroxyvitamin D3 ELISA Kit from Innovative Research is intended for the quantitative determination of 1,25-Dihydroxyvitamin D3 in biofluid samples, such as serum, plasma and other biological fluids. This is a competitive inhibition ELISA in strip well format with reagents for up to 96 tests . DHVD3 present in samples reacts with an anti-DHVD3 monoclonal capture antibody or antigen coated and dried on a microtiter plate. Following this, DHVD3 conjugated to biotin competes with the antigen in the sample to bind the capture antibody or antigen. Unbound conjugate and sample antigen is washed away and bound conjugate is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is inverse proportional to the concentration of active DHVD3 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of DHVD3. Detection Target: 1,25-Dihydroxyvitamin D3 Reactivit