17-Hydroxyprogesterone ELISA Kit

17-Hydroxyprogesterone ELISA Kit from Innovative Research is intended for the quantitative determination of 17-Hydroxyprogesterone in biofluid samples, such as serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. This is a competitive inhibition ELISA in strip well format with reagents for up to 96 tests . 17-OHP present in samples reacts with an anti-17-OHP monoclonal capture antibody or antigen coated and dried on a microtiter plate. Following this, 17-OHP conjugated to biotin competes with the antigen in the sample to bind the capture antibody or antigen. Unbound conjugate and sample antigen is washed away and bound conjugate is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is inverse proportional to the concentration of active 17-OHP in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions o