*Human Total tPA ELISA Kit

Select this product for Molecular Innovations name, Human tPA Total Antigen Sandwich ELISA Kit and product number, HTPAKT-TOT. This human tissue-type plasminogen activator (tPA) total antigen assay is intended for the quantitative determination of total tPA in human plasma and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. Human tPA will bind to the affinity purified capture antibody coated on the microtiter plate. Free and complexed tPA will bind to the plate. After appropriate washing steps, monoclonal anti-human tPA primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the peroxidase conjugated secondary antibody. Following an additional washing step, TMB is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human tPA. Color development is proportional to the concentration of tPA in th