*Human tPA Purified Non-Enzymatic

Select this product for Molecular Innovations name, Non-enzymatic Human tPA and product number, HTPA-ALA.Human tPA Purified Non-Enzymatic from Innovative Research has been recombinantly produced in insect cells and purified using affinity chromatography. This is a frozen liquid buffered in 0.4M HEPES; 0.1M NaCl; pH 7.4 and a purity of >95%. This product is useful as a positive control for ELISA and western blot and for a variety of in vitro applications including cell differentiation, cell signaling, clotting assays, fibrinolysis, plasminogen activation, protein-protein interactions, receptor binding, thromboelastography, and thrombolysis. This human tPA has an active site serine to alanine mutation which renders it catalytically inactive. The mutation site is S478A on the mature protein and S513A on the complete mRNA sequence (UniProtKB: locus TPA_HUMAN, accession P00750). It is >90% single chain and retains exosite binding as well as other properties of wild type tPA. More Deta