*Human tPA Purified Non-Enzymatic Non-Cleavable

Select this product for Molecular Innovations name, Non-enzymatic, non-cleavable human tPA and product number, HTPA-ALANC.Human tPA Purified Non-Enzymatic Non-Cleavable from Innovative Research has been recombinantly produced in insect cells and purified using affinity chromatography. This is a frozen liquid buffered in 0.4M HEPES; 0.1M NaCl; pH 7.4 and a purity of >95%. This product is useful as a positive control for ELISA and western blot and for a variety of in vitro applications including cell differentiation, cell signaling, clotting assays, fibrinolysis, plasminogen activation, protein-protein interactions, receptor binding, thromboelastography, and thrombolysis. Human tPA with a double mutation that is permanently single chain and lacks catalytic activity. The non-catalytic mutation site is S478A on the mature protein and S513A on the complete mRNA sequence (UniProtKB: locus TPA_HUMAN, accession P00750). The non-cleavable mutation site is R275E on the mature protein and R310