Soil Extract Medium

Soil Extract Medium General purpose freshwater medium suitable for axenic and xenic cultures. Modification of Bristol medium. Directions For 1 L Total Prepare 1 L of Bristol Medium and thoroughly mix. Discard 40 mL of the Bristol Medium. Add 40 mL of previously prepared Soilwater: GR+ Medium (Note: The GR+ should be filtered to remove soil particles]. For 1.5% (w/v) agar medium: add 15 g of agar into the flask; do not mix. Cover and autoclave medium. Store at refrigerator temperature. # Component Amount Stock Solution Concentration Final Concentration 1 Bristol Medium 960 mL 2 Soilwater: GR+ Medium 40 mL of supernatant