WC Medium

WC Medium Directions For 1 L Total To approximately 900 mL of dH2O, add the following components in the orer listed (not including the vitamins) while stirring continuously. Adjust the pH to 7.8 Transfer the contents of the beaker to a 1-Liter graduated cylinder and bring the total volume to 1 L with dH2O. Add the sterile vitamin components and mix well. Do not autoclave medium! Filter sterilize only. Store at refrigerator temperature. # Component Amount Stock Solution Concentration Final Concentration 1 NaNO3(Fisher BP360-500) 1 mL/L 85.1 g/L 1 mM 2 CaCl2•2H2O(Sigma C-3881) 1 mL/L 36.76 g/L 0.25 mM 3 MgSO4•7H2O(Sigma 230391) 1 mL/L 36.97 g/L 0.15 mM 4 NaHCO3(Fisher S 233) 1 mL/L 12.6 g/L 0.15 mM 5 Na2SiO3•9H2O(Sigma 307815) 1 mL/L 28.42 g/L 0.1 mM 6 K2HPO4(Sigma P 3786) 1 mL/L 8.71 g/L 0.05 mM 7 H3BO3(Baker 0084) 1 mL/L 24 g/L 0.39 mM 8 WC Trace Elements Solution 1 mL/L 9 Vitamin B12 1 mL/L 10 Thiamine Vitamin Solution 1 mL/L 11 Biotin Vitamin Solution 1 mL/L