WC+ Medium

WC+ Medium Component list is the same as WC Medium with the following exceptions: The addition of 1 mL of DYV Metal Solution Increase in the volume of Na2SiO3•9H2O Directions For 1 L Total To approximately 900 mL of dH2O, add the following components in the orer listed (not including the vitamins) while stirring continuously. Adjust the pH to 7.8 Transfer the contents of the beaker to a 1 L graduated cylinder and bring the total volume to 1 L with dH2O. Add the sterile vitamin components and mix well. Do not autoclave medium! Filter sterilize only. Store at refrigerator temperature. # Component Amount Stock Solution Concentration Final Concentration 1 Na2SiO3•9H2O(Sigma 307815) 2 mL/L 28.42 g/L 0.2 mM 2 NaNO3(Fisher BP360-500) 1 mL/L 85.1 g/L 1 mM 3 CaCl2•2H2O(Sigma C-3881) 1 mL/L 36.76 g/L 0.25 mM 4 MgSO4•7H2O(Sigma 230391) 1 mL/L 36.97 g/L 0.15 mM 5 NaHCO3(Fisher S 233) 1 mL/L 12.6 g/L 0.15 mM 6 K2HPO4(Sigma P 3786) 1 mL/L 8.71 g/L 0.05 mM 7 H3BO3(Baker 0084) 1 mL/L 24 g/L 0.39 mM 8 WC Trace Elements Solution 1 mL/L 9 DYV Metal Solution 1 mL/L 10 Vitamin B12 1 mL/L 11 Thiamine Vitamin Solution 1 mL/L 12 Biotin Vitamin Solution 1 mL/L